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Core Enzymes for COVID-19 Diagnostics

Hifair® V nCov Multiplex One-Step RT-qPCR enzyme mix

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As the COVID-19 pandemic continues to rage throughout the world, the demand for testing kits steadily increases. The availability of the testing kits, however, is often limited, which greatly impacts the effective control of the pandemic. Empowered by our innovative enzyme modification technology, large-scale protein fermentation/purification technology, and highly-efficient antibody screening technology, Yeasen has been playing an important role in the battle against coronavirus by providing enzymes for the production of millions of testing kits in China.

 

Yeasen’s Hifair® V nCov Multiplex One-Step RT-qPCR enzyme mix combines three major enzymes in one tube and allows sensitive quantification of RNA templates, including RNA viruses. Reverse transcription and PCR amplification are carried out in the same tube, simplifying hands-on operations and reducing cross-contamination risks. The enzyme mix is highly resistant to common PCR inhibitors with enzymes engineered to be thermostable and are compatible with the UDG enzyme-based anti-contamination system. We also provide single- enzyme products for major components within the enzyme mix, namely the Hifair® V Reverse Transcriptase, the UNICON® HotStart Taq DNA Polymerase, and the Murine RNase inhibitor. A ready-to-use reaction buffer is also available, which is optimized for the enzyme mix or the single-enzyme products.

 

Our UNICON® HotStart Taq DNA Polymerase is an antibody modified enzyme whose activity is blocked at room temperature and will only be restored after the antibodies are denatured and dissociate from the polymerase during the initial denaturation step of the PCR. This technology ensures high specificity and sensitivity of the PCR reactions, effectively reduces false positives and allows room-temperature reaction setup.

  

Hieff Unicon® Hotstart Taq DNA Polymerase exhibits high sensitivity and specificity

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Figure1. Amplification curve of ORF1ab and E gene using Hieff Unicon® Hotstart Taq DNA Polymerase

 

Hieff Unicon® Hotstart Taq DNA Polymerase exhibits excellent stability

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Figure2. Amplification curve of 10,000 copies of ASF plasmid using Hieff Unicon® Hotstart Taq DNA Polymerase. The blue curve represents the amplification after the reaction was setup and the complete mastermix was stored at 4℃ for 10 days,and the red curves represent the amplification after the reaction was setup and the complete mastermix was stored at 4℃ for 0 day

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Fgure3. Amplification curve of 10,000 copies of ASF plasmid using Hieff Unicon® Hotstart Taq DNA Polymerase. Blue: Amplification curve after the reaction was setup and the complete mastermix was stored at 37℃ for 5 days; Green: Amplification curve after the reaction was setup and the complete mastermix was stored at 37℃ for 3 days; Yellow: Amplification curve after the reaction was setup and the complete mastermix was stored at 37℃ for 1 day; Red: Amplification curve after the reaction was setup and the complete mastermix was stored at 37℃ for 0 day.

 

Our featured products related to COVID-19 diagnostics:

Applications

Product Name

Catalog No.

DNA direct amplification

Hieff UNICON® HotStart Direct Taq DNA Polymerase

10717ES

DNA amplification

Hieff UNICON®  HotStart Taq DNA Polymerase

10729ES

Double-Block anti-Taq antibody

Hieff® Double-Block anti-Taq DNA Polymerase Antibody

31303ES

Anti-Taq antibody

Hieff® anti-Taq DNA Polymerase Antibody

31301ES

Reverse Transcription

Hifair® V Reverse Transcriptase

11300ES

RNase inhibitor

Murine RNase inhibitor

10603ES

UDG/UNG

Uracil DNA Glycosylase (UDG), heat-labile

10303ES

LAMP

Bst II DNA polymerase

12908ES

 

 

Production Capacity:

YEASEN has a production capacity of up to 800L per batch of fermentation, and our daily production of core enzymes for Covid testing kits can enable the diagnostics of 1 million people. All our production processes are performed under stringent quality controls to eliminate batch-to-batch variation. With our adequate inventory, we can continuously provide you the products you need at reasonable prices! Contact us to learn more about our products and to get free samples to test out! (Please kindly note that YEASEN will not cover the shipping fees.)

 

About YEASEN:

YEASEN Biotechnology (Shanghai) Co., Ltd. was founded in 2014 and Headquartered in Shanghai International Medical Zone. With our continued dedication to developing and producing high-quality enzyme products, we have become one of China’s top brands in molecular biology enzyme manufacturing. We are able to offer a broad selection of enzymes and reagents for research and applications such as molecular and cell biology studies, next-generation sequencing (NGS), and in vitro diagnostics (IVD).

 

As a biotech company with a mission of contributing to the advancement of life sciences, YEASEN took quick actions to develop and produce products to help in this fight against coronavirus. We have reached strategic cooperation with many IVD companies in China. To date, ten companies are using our enzymes for the manufacturing permit application of their COVID-19 testing kits, and more than 20% of the COVID-19 testing kits in the Beijing area are using our enzymes as the core components. We are incredibly honored to make an impact in this battle and will continually provide high-quality products to assist the research, diagnostics, vaccination, and treatment of coronavirus.