V Universal Multiplex One Step RT-qPCR Probe Kit

Details:

Product Description

HifairTM V Multiplex One-Step RT-qPCR Probe Kit is for multiple quantitative PCR reactions with RNA as a template. During the experiment, the reverse transcription and quantitative PCR were carried out in the same reaction tube, simplified experimental operation and reduced the risk of pollution.

The kit uses heat-resistant HifairTM V Reverse Transcriptase to efficiently synthesize the first chain cDNA, and uses UniconTM Hotstart Taq DNA Polymerase to quantify quantitative amplification. The kit mainly includes an optimized MP Buffer, Enzymes Mix, etc., which already contains Mg2+ and DNTP, etc., and has added factors that effectively inhibit non-specific PCR amplification factors and enhance multiple qPCR reactive amplification efficiency, which can be guaranteed at the same time of primer amplification, up to four reactions.

Applicable Models

ABI Series: ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus; 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex;

Bio-Rad Series: Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Roche Series: Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96;

Others: Stratagene MX3000P, MX3005P, MX4000P;

Eppendorf Mastercycler ep realplex, realplex 2 s;

Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Thermo Scientific PikoReal Cycler; Cepheid SmartCycler; Illumina Eco qPCR.

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for one year. Please avoid repeated freeze-thaw. It is recommended to save.

Cautions

1) Please use the RNase-free consumables during the experiment;

2) For your safety and health, please wear lab coats and disposable gloves for operation.

COA Please fill out the COA application form

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