Heat-Labile UDG (uracil DNA glycosylase) catalyzes the release of free uracil from uracil-containing DNA by hydrolyzing the N-glycoside bond between uracil bases and sugar-phosphate skeletons. Compared with conventional UDG enzymes, heat-Labile UDG can avoid the degradation of dU-containing amplification products caused by the residual activity of the inactivated UDG at room temperature. Heat-Labile UDG (uracil DNA glycosylase) works at room temperature and is thermolabile and easy to be inactivated.
Applications
Enzyme | Glycosylase |
Compatible Buffer | Storage Buffer |
Heat Inactivation | 50°C, 10 min |
Unit Definition | One unit (U) is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C. |
Components No. | Name | 10303ES60 (100 U) | 10303ES76 (500 U) | 10303ES96 (10,000 U) | 10303ES97 (100 mL) | 10303ES98 (500 mL) |
10303 | Uracil DNA Glycosylase (UDG/UNG), heat-labile | 100 μL | 500 μL | 10 mL | 100 mL | 500 mL |
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years.
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