Product Description

Hieff NGS™ Ultima Pro DNA Library Prep Kit for Illumina® is a new generation library construction kit specially developed and designed for the Illumina® high-throughput sequencing platform. On the basis of the previous generation of library construction kit, this product shows higher efficiencies in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA.

1、 Suitable for 500 pg-500 ng of DNA, including FFPE, cfDNA etc.

2、 With an industry-leading library conversion rate of more than 70%.

3、 Multi-sample validation for superior library and sequencing data.

4、 Strict batch stability.

Product Components

Component Number and Name			12201ES24	12201ES96
12201-A		Endprep Buffer 2.0	144 μL	576 μL
12201-B		Endprep Enzyme	96 μL	384 μL
12201-C		Ligation Enhancer	720 μL	3×960 μL
12201-D		Novel T4 DNA Ligase	120 μL	480 μL
12201-E		Canace™ Pro Amplification Mix	600 μL	4×600 μL
12201-F		Primer Mix	120 μL	480 μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for one year.

Cautions

1 Operation

1.1 For your safety and health, please wear lab coats and disposable gloves for operation. This product is for research use ONLY!

1.2 Thaw components at room temperature. Once the components are thawed, mix thoroughly by vortexing, spin the tube briefly and place them on ice for later use.

1.3 When preparing the reaction solution of each step, it is recommended to use a pipette to mix well or gently shake. Vigorous shaking may cause a decrease in library output.

1.4 It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tips when processing different samples.

1.5 It is recommended to perform each reaction step in a thermocycler with a heated lid. The thermocycler should be preheated to the set temperature before use.

1.6 Improper operation is likely to cause carry-over contaminations through aerosols, impacting the experiment’s accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.