Monkeypox Virus Real Time qPCR Kit is a PCR kit for the detection of monkeypox virus. Contains monkeypox virus primer probes, using the company's new-generation antibody method hot-start Taq enzyme 2× Mix master mix reagent and positive control, including Mg2+ and dNTP, etc., and adding factors and enhancements that effectively inhibit non-specific PCR amplification The factor of the amplification efficiency of the multiplex qPCR reaction can improve the amplification efficiency of the reaction and promote the efficient amplification of low-concentration templates.
Component |
13862ES03(24 T) |
13862ES48(48 T) |
13862ES59(96T) |
|
13862-A |
2×TaqMan qPCR Mix |
300 μL |
600 μL |
1.2 mL |
13862-B |
Prime&Probe Mix |
24 μL |
48 μL |
96 μL |
13862-C |
MPV Positive Control |
120 μL |
240 μL |
480 μL |
【Notes】
a. 2×TaqMan qPCR Mix mainly contain heat-resistant UNICON® HotStart Taq DNA Polymerase, dNTPs, Mg2+, etc.
b. Prime&Probe Mix mainly contains target gene primer probes.
c. MPV Positive Control is a plasmid containing the target gene, the target gene is the FAM channel, and the concentration is 1×104 copies/μL.
The product is shipped with ice pag and can be stored at -20°C for one year. This product avoids repeated freeze-thaw. It is recommended to save.
Component |
Volume |
Final Concentration |
2×TaqMan qPCR mix |
12.5 |
1× |
Primer&Probe mix |
1 |
- |
DNA |
5 |
- |
ddH2O |
up to 25 |
- |
ABI Series: ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus; 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex;
Bio-Rad Series: Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;
Roche Series: Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96;
Others: Stratagene MX3000P, MX3005P, MX4000P;
Eppendorf Mastercycler ep realplex, realplex 2 s;
Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;
Thermo Scientific PikoReal Cycler; Cepheid SmartCycler; Illumina Eco qPCR.
Stage |
Temperature |
Time |
Cycles |
Pre-denaturation |
95°C |
5 min |
1 |
Amplification Reaction |
95°C |
15 sec |
45 |
60°C |
30 sec |
【Notes】:The annealing/extension temperature can be appropriately adjusted according to the experimental requirements.
|
FAM |
Result |
1 |
Ct>38 or UNDET |
Negative |
2 |
Ct≤35 |
Positive |
3 |
35<Ct≤38 |
Need to re-test, the Ct value is still in this range and the curve is in the standard "S" shape, it is judged as positive, otherwise it is judged as negative |
1.Please use the RNase free consumables during the experiment;
2.For your safety and health, please wear lab coats and disposable gloves for operation.
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