Description

DH5α Fast Chemically Competent Cell from YEASEN is a competeny cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with the conversion efficiency up to 10⁸ cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification, and can ensure stable inheritance of high-copy plasmids. At the same time, it can be used to construt clones, blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA. F-DH5α competent cells were prepared by special technology, without heat shock at 42℃ or incubated at 37℃, only 10 minutes to complete a transformation.

DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(r_k-,m_k+) supE44λ- thi -1 gyrA96 relA1phoA

Features

50. High transformation efficiency: ≥1×10⁸ cfu/μg pUC19 DNA
50. Genetic markers that allow for blue/white colony screening
50. Rapid: It only takes 10 minutes to complete one transformation

Applications

50. Efficient DNA cloning derived from PCR, cDNA-generation reactions
50. recA1 marker facilitates working with difficult to transform DNA

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether it contains an F’ episome	No
Efficiency	≥1×108
Blue/white colony screening	Yes
Bacterial or yeast strains	DH5α
Amplification of the ccdB-containing vector	It is not suitable for amplification of ccdB-containing vectors

Components

Components No.	Name	11803ES80
11803-A	DH5α Fast Chemically Competent Cell	10×100 μL
11803-B	pUC19 (control vector,10 pg/μL)	10 μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -85℃~-65℃ for six months. Do not store the product in -20℃ or liquid nitrogen.

 

                                                                                                                                                                                                                                                                                                                                                                                   Ver.EN20230928